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The filter is then sterilely transferred to the surface of a strong tradition medium (eg R2A medium well adapted to water micro organism). Quantitatively, the count of microorganisms present (expressed either in number of cells or in colony forming items per unit area or quantity) is performed based on completely different strategies to make sure compliance with specifications (ex. Bioburden is the population of viable microorganisms current on or in a product and / or sterile barrier system.

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